Journal: Kidney360

Article Title: Veverimer, a Nonabsorbed Gastrointestinal Tract HCl Binder, Decreases Renal Ammoniagenesis and Mitigates Nephrotoxic Serum Nephritis

doi: 10.34067/KID.0000000743

Figure Lengend Snippet: Urinary C5b-9–creatinine ratios in mice 2 weeks after NTS injection and plasma C3a concentrations 1 and 2 weeks after NTS injection with and without veverimer (V) administration. Two weeks after NTS injection, an approximately 80% increase in urine C5b-9–creatinine ratios was observed (compared with baseline values). Further indicating complement activation, NTS caused significant increases in plasma C3a concentrations at both the 1- and 2-week time points. When NTS-injected mice were treated with veverimer, both urinary C5b-9 ratios and plasma C3a levels were normalized, with values not significantly differing from those observed in normal urine samples ( n , 6 per assessment, ANOVA with Tukey HSD after test).

Article Snippet: Immunohistochemical staining for C5b-9 and CD45 were assessed as markers of complement activation and inflammatory cell infiltrates, respectively (performed by the Fred Hutch Cancer Center Experimental Histopathology Laboratory) using rabbit anti-mouse C5b-9 (Bioss bs-2673R; Woburn, MA) and anti-CD45 (Abcam ab10558), using a previously described methodology.

Techniques: Injection, Clinical Proteomics, Activation Assay

Journal: Kidney360

Article Title: Veverimer, a Nonabsorbed Gastrointestinal Tract HCl Binder, Decreases Renal Ammoniagenesis and Mitigates Nephrotoxic Serum Nephritis

doi: 10.34067/KID.0000000743

Figure Lengend Snippet: C5b-9 deposition, assessed by immunohistochemistry, 2 weeks after NTS injection with and without veverimer treatment. No evidence of C5b-9 deposition was seen in normal kidney sections (A). NTS caused heavy C5b-9 deposition throughout the histologic sections, as denoted by beige/light brown C5b-9 staining. This was accompanied by histologic evidence of tubulointerstitial nephritis with marked inflammatory cell infiltrates. A glomerular crescent is depicted (*). Conversely, C5b-9 deposits were not observed with veverimer treatment (B). Scale bars, 85 µ M.

Article Snippet: Immunohistochemical staining for C5b-9 and CD45 were assessed as markers of complement activation and inflammatory cell infiltrates, respectively (performed by the Fred Hutch Cancer Center Experimental Histopathology Laboratory) using rabbit anti-mouse C5b-9 (Bioss bs-2673R; Woburn, MA) and anti-CD45 (Abcam ab10558), using a previously described methodology.

Techniques: Immunohistochemistry, Injection, Staining